Programs that use GDAL as a back-end should be able to natively open .e00 files — for example QGIS, GRASS, SAGA, ogr2ogr, Python’s GeoPandas or R Statistical Programming’s sf package. This is a default built-in driver in GDAL.

If you have GDAL installed (which you may already have if you have any open source GIS software installed), you can also use ogr2ogr to do the conversion. Due to the limitations of Shapefiles, you it is better to convert to GeoPackage, as Arc/Info E00 files can have field names longer than 10-character long Shapefiles.

ogr2ogr output.shp input.e00 # note output comes before input

In the past, gdal didn’t have native E00 support but all recent versions do.

About the AVCE00 driver: https://gdal.org/drivers/vector/avce00.html

Programs that use GDAL as a backend: https://gdal.org/software_using_gdal.html

ogr2ogr man page: https://gdal.org/programs/ogr2ogr.html

Take a look at Spatial Reserves blog for some other options using an online converter if you don’t have the open-source stack installed your computer.